Coding

Part:BBa_K1769008

Designed by: Ho-Tsang Tsai   Group: iGEM15_NYMU-Taipei   (2015-09-18)

MtrB + LVA tag

MtrB outer membrne protein is very stable. In order to utilize MtrB as a sensor, we added a proteolysis tag at the 3' end of mtrB. MtrB with LVA tag might be degraded much faster so that the leakiness of this protein will be much lesser and it is more easy to utilize this protein as a reporter

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None